Averchenko K. Mechanisms of influence of redoх-active nanoparticles (ReVO4: Eu3+ and CeO2-x) on the bioenergetic processes in mitochondria

Object of investigation: mechanisms of influence of redox-active nanoparticles on the basis of rare earth elements on the bioenergetics processes in mitochondria of liver cells. Aim of work: determination of the basic mechanisms of influence of redox-active nanoparticles on the basis of rare earths elements - cerium dioxide CeO2-x and orthovanadates ReVO4:Eu3+ (Re = Gd, Y, La) on the basic parameters of mitochondrial activity - mitochondrial potential and processes of respiration and oxidative phosphorylation in liver cells. Methods of investigation: fluorescence microscopy, microspectrofluorymetry, fluorescence spectroscopy, spectrophotometry, chemiluminometry, polarography, computational kinetic model. Scientific novelty: data on the pro- and antioxidant activity of nanoparticles depending on the microenvironment properties and physico-chemical parameters of the particles have been obtained; differences in the mechanisms of penetration of nanoparticles with different sizes into the cells have been determined; the mechanisms of the influence of tested nanoparticles on the redox-processes in mitochondria have been proposed. Practical meaning: application in biophysics, molecular biology, pharmacology and biotechnology.