Mamuta O. Implementation of the femtosecond laser pulsed generation for diagnosis of the biological tissues and identification of the pathological growths (masses)

Thesis is dedicated to the research problem solving - development of the highly effective method for healthy (normal) and pathological tissues diagnosis that is based on femtosecond laser generation with varying wavelength. This problem was solved by development of the method for identification of the pathological tissues by four parameters: fluorescence spectra, average power of scattered and transmitted radiation, and temperature distribution at the depth of the biological tissues. The experimental setup for the optical and thermal characteristics investigation of the biological tissues under the action of femtosecond laser radiation was developed. The temperature distribution, the temperature gradients and the heat flow density were determined by the mathematical modeling and numerical analysis. The theoretical and experimental studies of the temperature distribution of the multilayer biological tissue, when it is heated under the influence of laser radiation, were verified. The experimental results of the optical characteristics investigation of the healthy (normal) and pathological biological tissues were presented. These results are the precondition for the development of the comprehensive approach, which will enable to assess the impact and to calculate the dose of laser exposure for laser therapy sessions, and also, will allow to identify the tissue pathology and to define precisely their bounds on the early stages, which is extremely necessary both for diagnosis and surgical intervention.