Kalachova T. Deciphering the role of phosphatidylinositol-dependent phospholipases and lipid kinases in plant response to environment stresses and developmental cues

Role of phosphoinositides and phosphatidic acid in the realization of biological action of phytohormones (salicylic and abscisic acid) during the establishing of plant defense responses and morphogenesis. Radioactive labeling, thin layer chromatography, DNA and RNA extraction and purification, Arabidopsis knock-out mutant lines, transcriptomic analysis, microscopy. The key role of lipid-derived second messengers (phosphoinositides and phosphatidic acid) was revealed in a regulation of suspension cells transcriptome by salicylic and abscisic acid. A particular involvement of phosphatidylinositol-dependent phospholipase C and diacylglycerol kinase 5 was shown in primary plant cells responses to bacterial elicitors and in pathogen recognition. An impact of phosphatidylinositol-4-kinase activity and the level of phosphorylated inositols at Arabidopsis root system morphogenesis and sensitivity to root growth regulators auxin and cytokinin was discovered and characterized. Implementation fields: bioorganic chemistry, biochemistry, biotechnology.