SHEIKH S. Immune status in Ukrainian children residing in contaminated area, with irritable bowel syndrome

Irritable bowel syndrome (IBS) is observed mostly in Ukrainian children and may be related to adverse health effects as a result of the Chernobyl disaster. The aim of this study was to determine the status of immune system in patient with IBS and dysbiosis of the gastrointestinal tract flora exposed to natural radiation with clinical symptom of IBS for effective diagnosis and treatment. Internal radiation activity was measured by gamma-ray spectrometry. Peripheral blood leukocytes were analyzed for B and T-lymphocytes subset such as T-lymphocytes CD3+, CD4+, CD8+, CD22+, CD16+ subset assayed by flow cytometric method, serum concentration of circulation immune complex was measured by the polyethylene glycol method. Phagocytic activity function was assessed by using latex article and phagocytic index were calculated. Serum immunoglobulins (A, M, G, E) and seum level of cytokine profile were evaluated by enzyme-linked immunosorbent assay (ELISA) method. The intestinal bacterial microbiota was assessed by medical microbiologic method witch proposed by Sutter. The effect of polyoxidonium on the correction of immune status was studied in vivo. A total number of 16 children with IBS and dysbiosis aged 14-18 years as a study group received polyoxidonium with basic therapy and 10 children with IBS and dysbiosis in same age received only basic therapy as a control group. The basic therapy is a number of different medications are used to help treat IBS, including antispasmodic medicines and antimotility medicines with pre- and probiotics supplements as like as Lacium.