Synetar E. Formation of biofilms by Enterococcus faecalis and Candida albicans strains on catheters and the search of anti-adhesive drugs.

The dissertation is devoted to the study of peculiarities of E. faecalis and C. albicans strains biofilms formation on the silicone and latex catheters, as well as the determination of the anti-aggressive activity of selenium citrate, silver nanoparticles and hibiscus extract. It has been established, that among the potential causative agents of UTIs in the examined patients, bacteria of the Enterococcus genus and yeast fungi of the genus Candida were predominant, and were excreted in urine from 58.7±5.2% and 23.9±6.3% of patients in the range of 105–107 CFU/ml respectively. Strains of Enterococcus sp. were resistant only to ciprofloxacin, levofloxacin and moxifloxacin. C. albicans strains were characterized by 100.0% susceptibility to antimycotics with the exception of flucytosine, to which 30.0±13.8% of strains were resistant. It was shown, that 81.0±5.7% of strains of bacteria, and 45.0±7.3% of C. albicans strains were characterized by high adhesive activity, as well as the ability to form biofilm on the surface of silicone and latex catheters in 48–72 h. The lower level of adhesion of E. faecalis and C. albicans cells, as well as the slower dynamics of biofilm formation, is recorded on the surface of the latex catheters as compared to silicone. Extreme anti-adhesive activity is characterized by subinhibiting concentrations of selenium citrate, hibiscus extract, as well as non-silver particles at concentrations of 0.01, 200.0 and 0.01 mg/ml, respectively.