Penteliuk O. Inner dendro diversity of Aesculus L. urban environment of Kyiv (adaptation to entomological stress, secondary metabolism, resistance)

The thesis presents the results of comprehensive studies of the on ecosystem, structural – functional and molecular – biological approaches for the first time on the theoretical, scientific methodical and experimental justification. The new solution proposed for the actual scientific problem concerning to the formation of adaptive reactions for the species of the genus Aesculus L. damage to C. ohridella of the Kyiv’s urban environment. The accumulation regularity established in content and composition of secondary metabolites, especially phenolic compounds in leaves of resistant species and cultivars of genuses’ plants Aesculus L. in conditions of water insufficiency. The scientific and methodological recommendations were developed and the experimental material on optimization of biotechnological stages of clinical microproliferation in vitro resistant to C. ohridella cultivar A. hippocastanum L. for planting in Kyiv for the first time. The ecobiocultures with unique ecological properties are took into the account as promising donors for obtaining somatic clones of stable tree forms during the process of visual exploration of the green plantations of Kyiv. The contribution of flavonoids evaluated for the protection system formation of A. hippocastanum L. cell structures from damage to C. ohridella. The biochemical markers were proposed for the average medium and high molecular weight compounds Rf from 0.1 to 0.3. They were received by chromatographic profiling for selection of seedlings of cultivar A. hippocastanum L. with signs of high potential physiological resistance against C. ohridella. It is advisable to use artificially awakened lateral and flower buds and shoots were took in the winter for the receiving aseptic viable actinus A. hippocastanum L. The explants use of leafy and flower meristems A. hippocastanum L. are chose in April – May from Kyiv’s isolated plantations. It is not effective way because they infected by epiphytic microflora. The microclone propagation of resistant C. ohridella to A. hippocastanum L. cultivar suitable for adaptation under conditions in vivo, methodological approaches and technology of phased indirect morphogenesis have been developed through the successive initiation of callus formation – dedifferentiation and differentiation of tissues with morphogenic structures.