Pohorila M. Immunomodulating properties of embryonic tissues of birds extract of 9 days of fetation with induced immunodepression

In the course of the work it was found that in the embryonic tissues of birds extract of 9 days of fetation the concentration of protein is 0,77±0,05 mg/ml, the level of FGFb - 120±6,8 ng/ml which were used as the criteria for its standardization. It has been found that a single ETE 9 produces a greater stimulating effect in vitro than individual hydrophilic and hydrophobic fractions: on indicators of synthesis of IL-2 by splenocytes and the phagocytic activity of macrophages of mice with cyclophosphamide immunosuppression. A single administration of ETE 9 to mice at a dose of 0,1 mg/kg a day before the action of γ-radiation increases the survival rate of mice for 30 days after irradiation at the dose range of 3 – 9 Gy. On the model of immunosuppression induced by γ-radiation at a dose of 5 Gy, it was shown that ETE 9 promotes accelerated recovery of bone marrow, thymus and spleen morphology in comparison with spontaneous reconstitution. The structure of the medullary tissue assumes a physiological state on the 14th day of observation. It was discovered that under the influence of ETE 9, the absolute number of bone marrow cariocytes and the population composition of its lymphoid compartment were normalized already on day 14, thymocytes and cariocytes of the spleen – on the 21st day, what is ahead of the reconstitution of indices under the influence of the «Erbisol». The level of products of lipids peroxidation in bone marrow cells reaches physiological values on day 14, as opposed to the group receiving «Erbisol». The acceleration of the normalization of absolute number of the formed elements of blood, which occurs on the 14th day, ahead of the reconstitution of similar indicators in the comparison group, is also recorded. Normalization of the proportion of CD3+, CD4+, CD8+ lymphocytes in groups using ETE 9 and «Erbisol» occurs simultaneously (at day 7), and the proportion of CD45R/B220-lymphocytes is on the 14th and 21st days, respectively. Under the influence of ETE 9, restoration of the functional activity of T and B-lymphocytes in animals with immunosuppression is achieved on average 7 days earlier than in the comparison group.