Bodnarchuk N. Changes in biophysical and morphological parameters of loach embryos for actions of flurenizyd

Dissertation is dedicated to the research of the free-radical reactions (in vitro and in vivo) of loach embryos for actions of flurenizyd – preparation of antimicrobial, antituberculousis, antichlamydia, immunomodulator, antioxidant, hepatoprotective, antiinflammatory, antiviral action. Influence of flurenizyd is studied on functioning of Na+, K+-АТPаse of loach embryos Misgurnus fossilis L. on the stages of development 2, 16, 64, 256 and 1024 blastomeres. Flurenizide in all studied concentrations (0.01; 0.05; 0.15; 1; 5; 15 mM) caused increase in content of lipid peroxidation primary products (hydroperoxides) during development of embryos but decrease on stage 256 blastomeres. The article contains data on ultrastructural and morphological changes of embryos of loach Misgurnus fossilis L., what developed in incubation environment, which contain flurenizyd. It is shown that an antibiotic is in the concentrations of 1; 5; 15 mМ predetermines the high death rate of embryos of loach already on the first time of his action. It is set that flurenizyd causes structural violations of mitohondria and cytoplasmic net, causes the increase of amount of lysosomes at the embryos of loach on the stage of development of 2 blastomeres. A degree of expressed of these changes is dosedependent. The obtained results extend understanding of the mechanisms of flurenisid action on membrane processes of embryonic cells. These data can be used for toxicological evaluation of bioactive or pharmacological substances, and therefore have value for human and veterinary medicine. After a two-factor analysis of variance for the first time found that the formation of primary products of lipid peroxidation (hydroperoxides) has a significant effect of loach embryos. However, the formation of secondary products of lipid peroxidation approximately the same effect commit time and flurenizyd and unmeasured factors. At superoxidedismutase, catalase, glutathioneperoxidase and glutathione-S-transferase activity of loach embryos exert a powerful influence unmeasured factors, which may include external factors in which development occurs. We found that the development is more pronounced effect on catalase activity. Applying cluster analysis was first found to be under 64 blastomeres for all experimental groups studied parameters were divided into 4 groups of similarity. Revealed that the concentration of flurenizyd 0,05mM not have a significant impact on performance, the value of which was at the level of control (at 2 blastomeres). At the stage of 256 and 1024 all blastomeres study group on indicators are divided into 5 groups of similarity that indicates a change flurenizyd impact on those stages of loach embryos. The research results are important for flurenizyd use in the treatment of pregnant women as well as for further research on its effects on reproductive function. The conclusions arising from the research results can be used in the formation of new regulations related to pharmacology.