Halunko H. Morphological changes in the small intestine in the late stages of burn disease and their correction by infusion solutions (experimental study).

An experimental study of the effect of infusion drugs on the structure of the ileum in later periods (14, 21 and 30 days) after burn skin lesions were performed on 147 laboratory white rats, males weighing 150-160 g. In rats after a thermal injury of the skin, which was injected 0.9 % NaCl solution, it was found that the most pronounced dystrophic and destructive changes in the small intestine wall are observed after 14 days of the experiment, which gradually decrease. However, even after 30 days there are areas of deformation and polymorphism of the intestinal villi. When administering infusion solutions lactoprotein with sorbitol or HAES-LX-5 % at all time periods after the burn injury of the skin, necrobiotic and dystrophic changes and leukocytic infiltration are less pronounced than in rats which was injected 0.9 % NaCl solution after burning the skin. In the analysis of cell cycle and fragmentation of DNA of mucosal cells of the small intestine it was first established that all infusion solutions do not change the parameters of the interval of SUB-G0G1, S-phase, G0G1 and G2 + M phases of the mucosal cells in rats without burning skin injury in 14, 21 and 30 days of observation. While using lactoprotein with sorbitol solutions or HAES-LX-5 % after 14 and 21 days after burning the skin, the S-phase and the proliferation index increased compared with the burn group + 0.9 % NaCl solution; after 21 days, the indicator of the interval SUB-G0G1 in both groups is lower than in the burn group + 0.9 % NaCl solution; after 30 days, using HAES-LX-5 %, all cell cycle indices have no significant or trendy differences compared to those in the non-burning group, and when using lactoprotein with sorbitol, the parameters of the G0G1 phase and the block of proliferation remain lower than those of the same indicator in rats without skin burns. Кey words: small intestine, rats, thermal burns of the skin, infusion solutions, micro and ultrastructural changes, cell cycle and DNA fragmentation. Branch-Medicine.