Yakoviv M. Amphiphilic fluorescein-containing copolyesters of N-derivatives of glutamic acid obtained by the Steglich reaction

The dissertation is devoted to the synthesis of amphiphilic copolyesters by irreversible polycondensation method, using Steglich reaction, based on natural amino acids, polyether diols and fluorescein and study of their structure, chemical-colloid and biochemical properties. Complex studies of surface activity of fluorescein-containing copolyesters in aqueous media, particle size and stability of their dispersions, depending on the nature of monomers, their ratio and content of the fluorescein in the copolyester were performed for the first time. It has been established that synthesized copolyesters are able to equally distribute between water and lipid phases, which is one of the main characteristics of the carriers for drug delivery systems. The aggregate stability of aqueous dispersions, sorption by particles of the disperse phase of albumin of bovine serum, patterns of hydrolytic degradation of copolyesters and their complexes with albumin and influence of these parameters on the composition and structure of copolyester were determined. Evaluated the ability of amphiphilic copolyesters to penetrate into living cells and the possibility to control this process, using the luminescence effect of fluorescein. The study of the effects of fluorescein-containing copolyesters on the functional and structural conditions of organs of laboratory animals made it possible to conclude that they are safe for the organism and can be used as independent biochemical markers, as well as in the drug delivery nanosystems for marking them and studying their properties.