Malimon Z. Scientific Substantiation of Microbiological of Imported Frozen Fish and Its Assessment on Antibiotic Residues

The thesis research directed to justify of microbiological criteria of frozen fish imported into Ukraine, and its assessment on antibiotic residues. The special attention is focused on process microbiological hygiene criteria which are based on enumeration of psychrotrophic microorganisms in frozen fish. It has been established that the psychrotrophic microflora of frozen fish is quantitatively dominated than the of mesophilic microflora in 1.3–2.0 times and more characterizes the sanitary conditions of the production process. It has been found that the enumeration of mesophilic microorganisms in 92.6±2.5 % samples of frozen fish was conformed to current requirements of the national standard. At the same time, in assessing such fish, the content of the psychrotrophic microflora in that samples were exceeded than 5×104 CFU/g and was in 2.6 times (p<0.05) more than the samples which were contaminated of mesophilic aerobic and facultative anaerobes microorganisms. It has been developed of process microbiological hygiene criteria which are based on enumeration of psychrotrophic microorganisms, which demonstrate to the compliance of a complex of hygienic measures from freezing to retail: n=5; c=3; m=10000 CFU/g; M=50000 CFU/g. It was found that most of the frozen fish imported into Ukraine had antimicrobials from the group of aminoglycosides I–II generation (46.7±0.7 %) and nalidixic acid (19.0±0.2 %) from total positive samples. It was found that imported frozen fish contented antibacterial substances, researches of which are not provided as in the state monitoring plan for residues of veterinary medicines, as in the Regulation EU 37/2010.