Mechanisms of oxidative stress consequence in animals were measured against the background of intoxication with copper ions after multiple successive exposure to cuprous sulphate in a dose of 1 μg / 100 g body weight.
In order to determine the role of pro-antioxidant system in the development of pathology, in particular, liver fibrosis, low molecular weight components of colostrum and a biologically active additive "mix-factor" that have a wide range of effects on biological systems, including those having antioxidant effect, were used.
It can be assumed that by effecting the pro-oxidant-antioxidant system, biologically active additives ("mix-factor" and low molecular weight components - "LMWC") can: eliminate the effects of intoxication with cuprous sulphate or increase the life expectancy of older animals that may have an increased number of free radical reactions as a result of aging.
The purpose of the work was to measure the mechanisms of regulation of some values of the pro-antioxidant system in animals after intoxication with cuprous sulphate and the influence of low molecular weight components of biological origin ("mix-factor", " LMWC ") on these values, as well as the interrelation of values of the pro-antioxidant system with physiological characteristics and life expectancy of experimental animals (rats).
The biochemical methods of research (activity of the pro-antioxidant system, enzymes, determination of biochemical parameters of liver activity (content of triacylglycerides, cholesterol, albumin, etc.)), as well as physiological parameters (temperature and body weight, working capacity of experimental animals) were used in this work.
It has been shown that repeated multiple consecutive administration of cuprous sulphate, at intervals of 48 hours, at a dose of 1 mg / 100 g body weight, which was 3 % of the lethal dose, was accompanied by weight loss, a decrease in body temperature by 0.5 +/- 1С°, decrease of working capacity. In these animals, the content of lipid hydroperoxides in both liver and blood serum mitochondria was increased by 45-90 % compared with control. In this case, the activity of glutathione peroxidase in both mitochondria and liver microsomes and serum was reduced by 35-40 %. The activity of γ-glutamyltransferase (GGT) was increased by 263 % compared with the control. However, such values of liver activity as the activity of alanine aminotransferase and aspartate aminotransferase remained within the control values. In animals after the intoxication with cuprous sulphate, the content of triacylglycerides, creatine, albumin remained unchanged. These results suggest that after the 3-fold administration of cuprous sulphate to experimental animals, an initial stage of development of liver fibrosis was detected and in the initial stages the indexes of the anti-oxidative system and GGT activity were primarily changed.
It was found that the low molecular weight components of colostrum, which are represented by amino acids, peptides and low molecular weight proteins, sugars and vitamins, had similar to the “mix-factor” effects on physiological parameters after intoxication of the organism. The obtained results allow us to conclude that the "mix-factor" and " LMWC ", despite the differences in the composition, cause similar physiological effects.
The introduction of the "mix-factor" and " LMWC " to experimental animals on the background of cuprous sulphate intoxication was accompanied by normalization or preservation of the amount of lipid hydroperoxides in serum, while their content against the background of intoxication was increased by 90 %. The activity of glutathione peroxidase, which was reduced, also corresponded to the norm after the introduction of the “mix-factor”, and after the introduction of “LMWC” to these animals, it even exceeded the level of control activity in the blood serum.
The “mix-factor” and “LMWC” normalized the content of lipid hydroperoxides in the liver mitochondria of animals after cuprous sulphate intoxication, which correlated with an increase in the activity of glutathione peroxidase in the blood serum of these animals.
The interrelation of the pro-antioxidant system with physiological indices can also be evidenced by data obtained from the influence of the “mix-factor” on the restoration of the level of hydroperoxides in serum and liver mitochondria and the activity of glutathione peroxidase at 22 and 30 months. animals up to the level of 12 months. animals and improving their quality of life (increased efficiency and reduced mortality).
The role of the redox system in the development of liver fibrosis and the possibility of its recovery after intoxication of the body with cuprous sulphate, “mix-factor”, and “LMWC” is shown.
