Dubovyk O. Sensitivity of skin purulent-inflammatory pathogens to complex action of antimicrobial preparations and LED radiation in vitro and in vivo.

The thesis is dedicated to the study of the impact of LED radiation and antimicrobial medications on planktonic cells and biofilms of microorganisms, purulent-inflammatory diseases pathogens. Practical solution of the actual problem is justified – action of blue and red spectrum of LED radiation and antimicrobial preparations on planktonic and biofilm forms of purulent-inflammatory skin diseases pathogens is determined on the assessment of microbiological, immunological and morphological signs. Etiologic factors of purulent-inflammatory skin diseases in children were stated. Isolates ability to biofilms formation and their sensitivity to antibiotics in plankton and biofilm existence forms were detected. The device for optical radiation impact on pathogen bacteria and method of LED radiation impact on microorganisms in vitro were worked out for experimental research. Optimal combinations for maximal oppression of daily biofilms formation and plankton cells isolates production with the use of chlorhexidine together with blue exposure and hydrogen peroxide with red exposure were detected. LED radiation of blue and red spectrum in combination with cefepime was proved to oppress plankton cells formation with further dense biofilms formation blockade and shorten focus of inflammation healing terms at local purulent-inflammatory infection up to 7 days compared with the use of antibacterial preparation alone.