Sarkis-Ivanova V. Antimicrobial Effect of Сhemotherapeutic Drugs and Physical Factors on Different Forms of Pseudomonas aeruginosa Population

The object of study is the viability, biological properties and biofilming ability of P.aeruginosa strains. The aim of the study is to increase the effectiveness of treatment of Pseudomonas aeruginosa infection and disinfection of surfaces contaminated with its pathogen on the basis of microbiological justification for selection and use of chemotherapeutic drugs, physical factors and their combinations with the greatest ability to inhibit Pseudomonas aeruginosa. Research methods: microbiological, biological, physicochemical, physical, mathematical and statistical. Theoretical and practical results: An antimicrobial composition for inhibition of biofilm formation of Pseudomonas aeruginosa has been developed. Available, safe and effective technology for the treatment of infected local radiation ulcers of the skin, the method of PDT. For the first time, the modes of operation of the electronic accelerator were determined, which provide bacteriostatic and bactericidal effect against test strains of Pseudomonas aeruginosa in model samples. The ecological safety of electron beam treatment of objects contaminated with Pseudomonas aeruginosa has been confirmed. Novelty: For the first time it was found that the minimum inhibitory concentration of antibiotics against bacteria in the state of biofilm formation increased 8-16 times compared to that determined for planktonic forms of test strains. For the first time, taking into account the data on the molecular mechanism of action, combinations of antibiotics and cationic polypeptides that have a synergistic antimicrobial effect and prevent the formation of biofilms of P.aeruginosa strains were determined. For the first time, the inhibition of the ability to form biofilms by P.aeruginosa strains by the combined action of a photosensitizer (methylene blue) and non-monochromatic photodiode irradiation has been shown. The parameters of bactericidal action of PDT components in relation to the reference and circulating strains of Pseudomonas aeruginosa were established - the use of 0.1% aqueous solution of methylene blue and irradiation with LED red light (λ = 630-650 nm) at an exposure of 30 minutes. For the first time it was experimentally established that PDT is effective against multidrug-resistant strains of Pseudomonas aeruginosa with a high ability to biofilm formation. It is determined that the antibacterial efficacy of PDT depends on the degree of virulence of microorganisms. New scientific data on the sensitivity of test strains of P. aeruginosa to different absorbed doses of relativistic electrons have been obtained. An almost linear relationship between the radiation dose and the decrease in the number of viable bacteria has been established. It is shown that the bacteriostatic aftereffect of the electron beam was observed at energy loads from 0.8 to 3.8 kGy. The bactericidal effect was observed after irradiation of test strains with doses starting from 4.0 kGy. The vector and strength of changes in the biological properties of strains have been elucidated for the first time P.aeruginosa under the influence of sublethal doses of relativistic electrons. Under the influence of sublethal doses of the physical factor, the suppression of the biofilm-forming ability of test strains of Pseudomonas aeruginosa by 1.7-6.6 times was determined. Degree of implementation: 1 patent; sectoral innovation, 8 acts of implementation. Scope (field) of use: health care (medicine).