Kurtova M. Optimization of the algorithm for serological diagnosis of allergic diseases in patients with polysensitization

Object of study: patients with seasonal allergic rhinitis and polysensitization. Objective: optimization of the algorithm for serological diagnosis of allergic diseases in patients with polysensitization. Methods: clinical, immunological and serological, statistical. Theoretical and practical results, scientific novelty: The epidemiological aspects of CCD-positivity and the influence of antibodies to the carbohydrate determinants on the population profile of sensitization of patients with allergic diseases were determined. Correlation between the number of positive reactions to the tested allergens and the percentage of patients positively reacted on CCD marker was found. The percentage of detection of antibodies to CCD was significantly increased in patients with 5 or more allergens markers on the blot. Polysensitized CCD-positive patients were recorded an increase in the percentage of positive reactions mainly to CCD-containing allergens, while CCD-negative patients we’re reported increase in the percentage of positive reactions to all allergens. Patients with anti-CCD antibodies significantly change the epidemiological pattern of sensitization in the population by increasing the percentage of positivity to plant and insect allergens and latex. Practical significance: For the optimization of serological examination of polysensitized patients, the algorithm based on the using of CCD-blockers was substantiated: patients with 5 or more positive reactions on the blot need re-examination with CCD blocker, which will overcome the problem of multiple sensitization caused by antibodies to the cross-reactive carbohydrate chains and optimized prescription of molecular diagnostics for the rational specific immunotherapy. Scope: in the practice of scientific research and educational process, for allergists, in the practice of immunological laboratories.