Miahka K. Scientific and Experimental Justification for Control the Safety and Quality of Natural Honey for the Residual Amount of Antibiotics

The dissertation presents the scientific and experimental substantiation of the control over the content of antibiotic residues chloramphenicol, florfenicol, neomycin and nitrofuran (AOZ) in natural honey. Analysis for the first time from 2012 to 2018 of the content of natural honey in two groups of substances – A6 and B1 (antimicrobial substances) and validated the limits of their detection. According to an analysis of the spread of bacterial diseases of bees, it is established that they are registered in 11 regions of Ukraine. The suitability of the enzyme-linked immunosorbent assay for the determination of residues of nitrofuran (AOZ), chloramphenicol, neomycin and florfenicol in natural honey samples with validation characteristics has been established. Enzyme-linked immunosorbent assay for the identification of florfenicol in honey was improved by using distilled water and ethyl acetate extraction to obtain accurate estimates of the high percent return (96.5 %) of florfenicol in honey, which provided high test efficiency. The dynamics of physico-chemical indicators of linden honey depend on the method of treatment of bee families with antibiotics, which is confirmed by the presence of statistically significant difference between the obtained values for 10 days and during storage after 30 and 120 days. At the same time, honey samples tested for organoleptic and physico-chemical parameters met the requirements of DSTU 4497:2005 «Natural honey. Specifications», except for the mass fraction of water and the mass fraction of sucrose for feeding the syrup compared to the aerosol treatment of bee families and the control group. The residual antibiotic content of linden honey after 10 days, and after storage (30 and 120 days) at 25 ºC in a dark place, is probably higher for syrup feeding than for aerosol treatment of bee families. After 120 days, the storage of linden honey significantly (p≤0.05) decreased by 15.4 % the content of chloramphenicol for aerosol treatment and florfenicol – (p≤0.05) by 13.6 % for the syrup feeding. Storage of linden honey for 4 months does not affect the presence of antibiotic residues in it.