Object of research: samples of biological material (feces) that were subjected to analysis for detection of C. difficile, collected from patients with CDI symptoms (presence of diarrhea after antibiotic treatment start or other “risk group” agents, symptoms of intoxication, intestinal bleeding, endoscopic features of PMC), reference and clinical strains of C. difficile, nutritive media for isolation and study of toxin-producing properties of C. difficile. Purpose of research: Increase of efficacy of laboratory diagnostics of enterocolitis caused by С. difficile through improvement of bacteriological methods of agent isolation and determination of its toxin producing properties. Research methods: bacteriological (isolation and identification of C. difficile isolates, study of their morphological, tinctorial, cultural, biochemical properties; antibiotic sensitivity; determination of the features of the dysbiotic changes of the intestinal microflora in patients with suspected CDI; study of the efficacy of different schemes of C. difficile isolation from biological material), biological (determination of toxin producing properties of C. difficile strains), mathematical and statistical (statistical analysis of the obtained results). Equipment: microscope Micromed-1 (St. Petersburg, Russia); thermostat TS 80-М-3 (Odesa, Ukraine); biological safety box, II class (ESCO, Singapore); APІ Rapid ID 32A (bio Mérieux, France); GEN box anaer (bio Mérieux, France). The thesis is devoted to the solution of the scientifically practical task that consists in optimization of approaches to the bacteriological diagnostics of diseases caused by toxin-producing strains of C. difficile. A nutritional medium for C. difficile cultivation with new composition was developed and patented, that has high growth properties and selective action compared to the analogues, and, due to the optimal transparency and density, is suitable for toxin detection with the help of reaction of specific immune precipitation with antitoxin. A scheme of bacteriological diagnostics of CDI was improved, which allows isolation of toxin-producing C. difficile strains. Data concerning the changes in intestinal microflora in CDI patients were supplemented. It was established that the isolated clinical strains of C. difficile preserve high sensitivity to the agents of choice – metronidazole and vancomycin, which allows to consider them as therapeutical agents of the first order in case of etiotropic treatment selection and prescribe them to patients based on the level of risk of the recurrent course of the disease. Toxin-producing properties of the clinical isolates were studied and compared to the museum strains of C. difficile. The extend of implementation: 5 acts of implementation, 1 patent. The field (industry) of use: public health (medicine: microbiology, immunology, infectious diseases).
