Zharikova D. Polymorphism at loci associated with E genes in Ukrainian soybean (Glycine max (L.) Merr.) varieties and lines

The dissertation is devoted to the study of the alleles of photoperiodic sensitivity genes E1, E2, E3, E4, E7 in genotypes of soybean varieties and lines that have been created in different breeding centers of Ukraine, and to evaluation of the influence of alleles of E genes on agronomic traits of soybean plants that have grown in Right Bank Ukraine. Molecular genetic analysis by using microsatellite and allele-specific markers permited to reveale polymorphism in microsatellite loci associated with photoperiodic sensitivity genes E1, E2, E3, E4, E7 and E3 E4 genes for 22 varieties and 29 soybean lines, among which 19 lines created by crossing, 10 lines created using chemical mutagenesis. In the studied material, by genotyping at MS loci Sat_038, Satt100, Satt229, Satt319, Satt354, Satt365 we have shown a high level of genetic polymorphism; there were detected 28 alleles (4,7 alleles / locus), that indicated a high genetic diversity at microsatellite loci linked with E genes. According to our results, the panel of these six MS-markers can be proposed for the differentiation and passportization for modern ukrainian soybean varieties. PCR analysis with allele-specific markers for alleles of E3 and E4 genes showed that most of ukrainian soybean varieties and lines are characterized by dominant alleles for E3 gene 70% and 75% for E4 gene.