Ryzhko I. Connective tissue cell reactions and mechanisms of systemic regulation of regenerative processes in local thermal injury on the background of hyperglycemia.

The dissertation is devoted to the solution of a topical scientific issue in modern cell biology – the study of features of connective tissue cell reactions and mechanisms of systemic regulation of regenerative processes occurring in response to a local thermal injury in order to develop the concept of neoangiogenesis regulation via the "connective tissue - bone marrow - peripheral blood" regulatory chain in healing of burn wound and its changes in experimental hyperglycemia. It was found that in burn wound healing that occurs under physiological conditions, transcriptional factor NF-κB acts as an inducer of the "connective tissue - bone marrow - peripheral blood" regulatory chain, which provides regulated angiogenesis. pNF-κB induces the production of myeloid progenitor growth factors in connective tissue cells, which stimulates the differentiation of neutrophilic granulocytes in the bone marrow and their release into the blood. Neutrophils enter the damaged area and cause the expression of VEGF and Flt-1 receptors in the connective tissue regenerate. In hyperglycemia, the migration of neutrophils into the connective tissue regenerate decreases; the expression of VEGF and Flt-1 angiogenic cytokines is inhibited, that leads to pathological changes in blood vessels, impedes the physiological healing, delays and disrupts the restoration of connective tissue architecture and may be an important factor in impaired tissue regeneration in diabetes.