Palagina I. Influence of the disperse azo dyes on the nucleic acids and proteins biosynthesis, with consideration of their long-term effects

Object of study: liver, testicles, bone marrow and blood of the rats. Aim of study: analysis of the shiftings in the nucleic acids and proteins under the disperse azo dyes (DADs) influence with special emphasis on the long-term effects. Methods of study and equipment: biochemical (definition of both the DNA, RNA, histone, non-histone and globuline proteins (HP, NHP, GlP) biosynthesis intensity and their concentrations), genetical and morphological; Beta-1 radio-biochemical device, spectrophotometer, photocolorimeter, refrigerating type centrifuge. Theoretical and practical results, novelty: has been shown that the azo dyes' short-term influence causes diverse changes of the DNA, RNA and proteins biosynthesis with the breach of the correlation between the dynamics of these processes. DNA- synthesizing activity of the nuclei is oppressed with its future stimulation, HP biosynthesis changes in the opposite directions, NHP and GlP synthesis is inhibited. Chronic injection of the DADs causes activation of the DNA, RNA and HP biosynthesis. The most essential shiftings in the biosynthesis are fixed in the liver, the lesser in the testicles and are not found in the bone marrow. All these changes proved to be connected to the DADs mutagenic and gonadotoxic effects. The exposed criteria of the genotoxicity have been used for the hygienic standardization of the DADs. Sphere of implementation: toxicological biochemistry, general toxicology, hygiene.