Andriyenko O. Evaluation of toxigenic potential of Stachybotrys chartarum (Ehrenberg : Link) Hughes

The dissertation is devoted to studying and evaluation of Stachybotrys chartarum toxigenic potential. This fungi are wide spread in nature and develop on substrates rich with cellulose. They are available to produce mycotoxins, which are causal agents of stachybotryotoxicosis — heavy disease of people and animals. Stachybotryotoxin preparations from 47 Stachybotrys chartarum strains have been obtained and evaluation of their biological activity have been carried on using the complex of test-systems. The ability of stachybotryotoxins to suppress the cyanobacteriae growth has been shown for the first time. K. marxianus 724 and 726 can be recommended for practical using in veterinarian laboratories for the evaluation of toxically-sanitary safety of forages and stachybotryotoxicosis preventive measures. On the model strain 13959а it was shown that high level of biomass and stachybotryotoxins accumulation was on medium balanced on nitrogen and carbon, in the conditions of intensive aeration of medium, an d with adding of microelements mixture. It was established that during the fungal growth aldolase activity increased while that of succinatedehydrogenase decreased proportionally to accumulation of stachybotryotoxins in medium. The method of isolation, purification and fractionalization have been improved. This It consists in additional purification of preparation from the lipids and pigments following with fractionalization of preparation by column chromatography method using the solvents systems in order of their polarity increasing. The partial identification of some components of S. chartarum 13959а toxic metabolits complex. The ability of this strain to produce roridin H — macrocyclic trichothecene characteristic for Dendrodochium and Myrothecium representatives — have been established for the first time.