Melnychuk S. Molecular mechanisms of carbon dioxide on development and support of animal hibernation state

Objects for the research: rats, gophers (Cittelus suslicus Gueld), fish (Cuprinus carpio), separated organs, blood, liver tissue, bull sperm, cell compartments, intermediates. Target of the research: to determ impact of molecular-biochemical mechanisms of the main forms of carbonic acid (рСО2 і НСО3-) on the main parts of exchange processes within dynamics of development, keeping and going out of the state of natural hypobios, as well as based on the results of molecular-biochemical and clinical investigations to develop models and effective ways for implementation the artificial hipobiosis state for biological objects to solve important problems in the fields of animal sciences and medicine. Methods of the research: molecular-biochemical, radioisotopical, spectrophotometrical, chromatographical, ensimatical, potentiometrical, cryoscopical, phisico-chemical, ultracentrifugical, etc. Practical results: filling in the correctives into the widely-known formulae of calculations for Redox state inside cells compartments while in the state of animals' natural and artificial hipobiosis with СО2 correction; improvement of the known carbonic acid method to preserve bull's sperm; working out the method fish artificial hipobiosis; the method of animal artificial hipobiosis as an alternative to general organism narcosis. Priority of the research is proved by four patents of Ukraine, one positive resolution on issuing the patent of Ukraine, and practical recommendations. The sphere for implementation: medicine, veterinary medicine, animal science.