Patyka T. Вacіllus thurіngіensіs in the microbiological control of number of insects

Українська версія

Thesis for the degree of Doctor of Science (DSc)

State registration number

0510U000102

Applicant for

Specialization

  • 03.00.07 - Мікробіологія

05-02-2010

Specialized Academic Board

Д.74.844.02

Essay

In dissertational work results of researches are stated and the complex characteristic of useful biological features new strains Bt different serotypes (Н1, Н10 and Н14) within the limits of manufacture and application of ecologically safe preparations for the microbiological control of number insects pests, directed on restoration and support biocoenotic balance in agroecosystems is given. It is shown, that selection strains Bt are active producers entomotoxines: high level exotoxicogenic and entomocyde activity strains BtН1 and BtН10 concerning contact generations Leptinotarsa decemlineata Say., Musca domestіca L., larvicidal activity strains BtН14 rather Aedes aegypti. It is established, that development of infectious processes and their intensity are in direct dependence from virulence and contagiousness microbe pathogen, and also from specific and macroorganism specific features. For the first time the phylogenetic analysis serovariants entomopathogenic bacteria Bt is carried out. At comparison nucleotide sequences on 16S rRNA existence strains polymorphism in group entomopathogenes ВtН1, ВtН10, ВtН14 which is connected from them entomocyde activity is established. Are developed methodological approaches of biotechnological reception of microbic preparations on the basis of perspective strains Bt with industrial-valuable properties and taking into account features microbe producers, and also special-purpose designations. The new methodical direction of an estimation of formation possible population entomoresistance concerning bacteria Bt and to the preparations created on their basis is created and the estimation of multilateral action of bioagents Bt on insects pests (antifidant action, metatoxic) is given. Scientifically-practical workings out are recommended and can be used for development of large-scale and regional systems of manufacture of biological products on the basis of Bt, for application of microbiological products with different mechanisms of action in agrocenosis as ecologically priority control devices of number of populations insects pests.

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