Koval' H. Microbial investigation of diagnosis, treatment, prevention of modern Klebsiella sapronosis infection

Object of study - reference and clinical Klebsiella strains, isolated from the environment and circulating in medical institutions. Purpose of study - on the basis of the available data on Klebsiella's etiological role in human pathologies and genesis of sapronoses, and biological properties of Klebsiella genus bacteria, to develop diagnostic bacteria preparations of selective anti-Klebsiella activity, and substantiate the tactics of reasonable use of antimicrobial preparations. Research methods. polymerase-chain reaction, dendritic cells methods of histological (electronic microscopy), immunological, experimental clinical and statistical research. Practical significance of the obtained results. As a result of our study, new methods of prevention and treatment of human Klebsiella infection have been developed and suggested. Anti-Klebsiella efficiency of the bacilli-based highly specific preparation has been proved; its pre-clinical tests have been conducted. Recommendations on prevention of heightened circulation of Klebsiella's clinical strains and other enteric bacteria have been developed with regard to antiseptic preparations, for infectious and non-infectious diseases hospitals. An anti-Klebsiella target vaccine has been substantiated and developed. Novelty. For the first time in Ukraine, a broad-scale comparative analysis of biological properties of a number of Klebsiella strains isolated from different environmental objects and circulating in medical institutions was conducted on factual material at regional level. For the first time, adequate experimental models of Klebsiella infection were developed: pulmonary, intestinal and septic forms. With the aid of methods of electronic microscopy, efficiency of a bacilli-based biopreparation to prevent and treat antiklebsiella infection was proved. For the first time, immune-modulating properties of Klebsiella's mutant strain as a potential basis of an anti-Klebsiella vaccine biopreparation were studied. In the study, theoretical conceptions regarding the phenomenon of Klebsiella and sapronotic infections polybiotrophy have been formulated. The results of the study have been introduced to Medical Microbiology, immunology curriculum Medical Universities. Medical microbiology, immunology.