Popovа V. Preclinical diagnosis and clinicoimmunologic aspects of type 1 diabetes mellitus

Object of the study: type 1 diabetes mellitus (T1DM) in children and adolescents. Aim of the study: elucidation of the immunological mechanisms of T1DM pathogenesis at different stages of disease progression and development of new approaches to preclinical diagnosis of disease in young persons. Methods and equipment: clinical, biochemical, hematological, radioimmunological, immunofermental, cytological; flow cytometry, statistics; FACStar plus, JEM-100C. Theoretical and practical results: immunological mechanisms of T1DM development at different stages of its formation in young persons were established.Role of decreased natural and dysfunction of adaptive immunity in mechanisms of T1DM development was proved. Advisable use of combined meeting and values of simultaneously elevated titers of IA-2A+GADA as prevailed combination of DAAb for prognosis of T1DM development was substantiated. New method for differentiated diagnosis of different preclinical stages of T1DM development was developed on the basis of determining DAAb level in blood plasma. Novelty:for the first time, the prospective complex clinicoimmunologic examination of preclinical state of T1DM development in children of the Ukraine was realized. The register of DAAb+ children was formed and the risk of T1DM development in them was determined. Advisable combination of using metabolic, hormonal and biochemical markers in children and adolescents for identifying the stages of T1DM development was substantiated. Degree of implementation: 4 declarative patents were obtained, results of studies are used in hospitals of practice and research profile of Kiev and other Ukrainian regions. Area of using: endocrinology, pediatrics.