Safronova L. Probiotic properties of bacteria of Bacillus genus and interaction of the preparations on their base with macroorganism

The dissertation is devoted to investigation of probiotic properties of bacteria of Bacillus genus and interaction of the preparations based on their with macroorganism. Accurate taxonomic position of the strains Bacillus sp. UCM B-5139 and UCM B-5140 was determined. Their membership of the species B. amyloliquefaciens ssp. plantarum was substantiated. Antimicrobial characteristics of the strains UCM B-5139 and UCM B-5140 was examined.Ability of the strain UCM B-5140 to synthesize antibiotics surfactin, fengycin, bacillaene, macrolactin and difficidin, and also siderophore bacillibactin was established. It was demonstrated that the strains UCM B-5139 and UCM B-5140 of the bacteria B. amyloliquefaciens ssp. plantarum synthesize the complex of bacteriolytic and yeast-lytic enzymes, extracellular amino acids and exopolysugars. From supernatans of culture medium of the strains the complex enzyme preparations were isolated possessing broad spectrum of protease activity: fibrinolytic, elastase, collagenase and caseinolytic. In the experiments in vivo the effect of veterinary preparation "Endosporin" on immune reactivity indices of macroorganisms was studied. Endosporin facilitates the increase of macrophages and lymphocites activity, normalization of immune system status in animals with dysbacteriosis, it is also characterized by antitumor properties. Probiotics' effect for medical practice of biosporin and subalin was investigated. In the experiments in vivo the ability of the preparation "Subalin" to increase the effect of anti-tumor vaccine was demonstrated. Safety and efficiency of biosporin for treatment of children with perinatal pathology for correction of disorders in intestinal microbiocenosis was proved. Ability of biosporin to restore microbiocenosis content of oral cavity and distal intestine, to reduce the severity of inflammation signs in mucous membrane of oral cavity and recurrences rate in complex treatment of children with recurrent aphthous stomatitis was demonstrated. Application on subalin for correction of endogenous intoxication syndrome in treatment of children with chronic hepatitis was substantiated.