Object of research: autosimbiotic strains of the Aerococcus genus as an integral part of human and animal microbiocenoses. Subject of research: properties of autosimbiotic strains of the Aerococcus genus, isolated from different locus of the human body and animals, as the basis for the autoprobiotics creation. Methods of research: microbiological (cultivation of microorganisms, identification, study of culture, antagonistic properties, sensitivity to antimicrobial drugs), microscopic (study of morphological, tincorial and adhesive properties), physiological-biochemical, statistical. Equipment: torsion weights ВТ-500, spectrophotometer СФ-46, thermostat ТС-80, autoclave ВК-75, light microscope Leica СМЕ, light microscope with phase-contrast nozzle Primo Star iLED. The problem of the A. viridans symbionts of different biotops of the macroorganism combination role, their biological properties, quantitative and qualitative indices was first introduced and scaled up which allowed them to be attributed to the normal microflora. A comparative estimation of biological properties (morphological, tinctorial, culture, biochemical, antagonistic, adhesive, antitoxic) of the various biotop symbionts of the Aerococcus genus has been carried out. The isolated from different biotopes aerococci population was divided into 4 biotypes by sensitivity to antibiotics, auxotrophic and biochemical properties. The primary barrier role of A. viridans symbionts against opportunistic microflora from the same biotopes has been established. The high adhesiveness of A. viridans autosimbiotists in the conditions of contact with epithelial cells and erythrocytes of the host and lower activity in hetero-adhesive conditions was demonstrated for the first time. The phenomenon of A. viridans translocation in the norm and in violation of the barrier function of the intestine was studied first. The necessity of the microorganisms translocation studying in the use of drugs with autosimbiotic strains is shown. The efficacy, safety and tolerability of Aerococcus autosymbionts are proven on experimental models of animal infections and preclinical trials. The influence of Aerococcus genus autostrains on the quantitative and qualitative indices of a normal human microflora was studied and their harmlessness was demonstrated when administered orally in laboratory animals. For the first time, the use of multi-biotic Aerococcus genus autostrains as the basis for the personified method of dysbiotic states correction was substantiated. The method of cultivating and visualizing the biochemical activity of A. viridans autosimbionts with the use of selective factors has been improved. Selective antibacterial complex with the definition of optimal doses for the autoprobiotic substance on the basis of A. viridans symbionts production was chosen. A biological test for the microbiocenosis status diagnostics was proposed, based on the determination of quantitative and qualitative indicators of the autosimbiotic aerococci presence in different macrophage loci. The method of aerosols prolonged storage in agar gel was developed, that can be used in the process of autoprobiotics creation and use. A collection of selected Aerococcus genus symbionts was created. Degree of introduction: introduced (1 patent of Ukraine to utility model No. 106478, 1 patent of Ukraine for invention No. 114242). Sphere (industry) of use: health (medicine).
