Kyryliv B. Species, Ontogenetic, Organ and Tissue Related Specificities of Protein Metabolism and the Activity of Hydrolytic Enzymes in Poultry as Influenced by Alimentation Factors.

Українська версія

Thesis for the degree of Doctor of Science (DSc)

State registration number

0519U000610

Applicant for

Specialization

  • 03.00.04 - Біохімія

05-06-2019

Specialized Academic Board

Д 35.368.01

Institute of Animal Biology of NAAS

Essay

Modern highly productive crosses and lines of poultry are particularly sensitive to the negative impact of technological and stress factors, which result in certain metabolism and physiological functions deviations. That is why systemic research aimed at determining the physiological and biochemical specificities of poultry growth and development is relevant, as it will offer a possibility to develop methods of metabolism regulation and improve the quality of products (eggs and meat). The thesis is dedicated to the study of protein metabolism intensity and hydrolytic enzyme activity in the digestive tract organs of different types of poultry during critical periods of ontogenesis with respect to species, age, type of productivity, physiological condition under the influence of alimentation factors. To fulfil the tasks set out in the paper, three series of comprehensive studies have been conducted (7 tests). The first test, run on High-sex brown egg-laying chicken during the period from 1 to 150 days old (on the premises of Berkut agrocompany located in Drohobych district of Lviv region), revealed regularities of metabolic processes in birds under study as compared to numbers indicated in passport for the cross. To correct the metabolic processes in poultry, a study has been conducted on the impact of adding 0.2% sodium sulfate and Natuzyme polyenzyme feed (second test) to the complete feed of egg-laying chicken as well as adding Kremnevit preparation in different amounts (third test). In the course of said series of tests, metabolism activity has been studied in chicken aged 1, 6, 35, 60 and 90 days and chicken aged 120 days (before egg laying) and 150 days (peak productivity). Chicken were slaughtered during said periods. The material used for research included tissue of muscular stomach cuticle, granular mucosa, duodenal mucus and chyme, pancreatic and liver tissue. In addition, we assessed economic and zootechnical indices that characterize productivity and biological value of chicken product (body mass, average daily body mass increase, egg-laying properties as well as morphometric indices and chemical composition of eggs). During the next two tests of the series run on Pharaoh quails, we compared metabolism intensity and productivity of poultry on the premises of Zhaivir-Agro LLC with passport indices and determined the impact of Bilo-Activ supplement on protein metabolism, hydrolytic enzyme activity, intestinal microbiocenosis and poultry productivity.

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