Trubka I. Clinical and experimental justification of the primary prevention of dental caries and chronic catarrhal gingivitis in school-aged children

Clinical and experimental justification of the primary prevention' optimization of dental caries and gingivitis in school-aged children was submitted within the dissertation research. Cariogenic model of experimental dental caries in rats was designed. The experimental model of associated progression of dental caries and gingivitis in rats was designed. Major pathogenetic mechanisms of the associated course of dental caries and gingivitis, which are manifested by enamel and dentin demineralization detected histochemically and gingival inflammation detected histologically were studied. The use of the mineral water containing Ca2+ and use of L. reuteri strain-based probiotic by 20.0 % 40.0 % reduces the expectable dental caries incidence in rats on the cariogenic model, and by on the associated progression model of dental caries and gingivitis. It was identified that children with the developing cariogenic environment and compromised colonization resistance in the oral cavity are exposed to antioxidant defense overstressing and failure, while the progression of dental caries and chronic catarrhal gingivitis (CCG) becomes associated. Clinical features of the associated progression of dental caries and CCG in school-aged children were identified, in particular, expectably higher values of dental caries severity, proportions between moderate and major severity of CCG. It was defined that the associated progression of dental caries and CCG expectably more frequently occurs in patients with genotypes 3953СТ+3953ТТ on the gene IL-1b, while the appearance of genotype 3953СС in children on the gene IL-1b is indicative of protective effects relating to the development of this associated tooth pathology. Data monitoring of clinical examination, biochemical properties of the oral fluid, identified periodontal pathogens in children aged 7-11 and 12-15 under the use of HPCs indicates the prevention of the further dental caries development, improvement of hygienic and periodontal indices, elimination of manifestations and prolonged remission, which take place due to the expectable elimination of periodontal pathogens. Research findings prove that implementation of family approaches to prevention and treatment of dental caries and periodontal diseases children with their parent's involvement.