Kozub N. Diversity and effects of of prolamin gene clusters in Triticum aestivum L. and related species

The thesis is devoted to the study of the diversity of prolamin gene clusters (alleles at prolamin loci) in Triticum aestivum L. and its cultivated and wild relatives and the effects of certain prolamin alleles. Based on the analysis of collections of genotypes of T. aestivum, hybridological analysis, mutagenesis, development of hybrid material with introgressions, including from crosses with Aegilops biuncialis Vis., we identified a number of new alleles at prolamin loci, in particular those resulting from intralocus recombination, mutations, and introgression.. А new secalin locus Sec-N was mapped distally from Sec-1. Several new 1BL.1RS translocations were identified and lines with recombinant 1RS arms involved in 1BL.1RS and 1AL.1RS were developed. The allele composition at the prolamin loci and diversity indices were determined in Dasypyrum villosum (L.) Candargy and Ae. biuncialis from the populations of the Crimean Peninsula. In Ae. biuncialis, we identified 11 alleles at the Glu-U1 locus, 19 at Glu-Mb1, 21 at Gli-U1, and 12 at Gli-Mb1. The genetic collection Ae. biuncialis with respect to alleles at the storage protein loci Glu-U1, Glu-Mb1, Gli-U1, Gli-Mb1 was developed and registered in the National Center for Plant Genetic Resources of Ukraine. The collection represents from 47% to 100% diversity of alleles at these loci in the Ukrainian populations of Ae. biuncialis.