Hruzevskyi O. Influence of local factors of colonization resistance, immune status and condition of neurohormonal regulation on the development and progression of bacterial vaginosis

The object of research: scrapings of the vaginal mucosa, vaginal contents, blood of persons who consulted a gynecologist for a preventive examination or complaints of discomfort in the genital area of varying severity. The purpose of research: to increase the effectiveness of diagnosis and prediction of bacterial vaginosis by determining the impact of neurohormonal regulation, immune status, and local factors of colonization resistance on the biocenosis of the vagina. Research methods: bibliosemantic (study of modern literature on the problem); general clinical (analysis of patients' complaints, medical history, results of clinical examination and features of chronic inflammatory processes of the female genital area); microscopic (study of morphological and tinctorial properties of bacteria in stained preparations; determination of "key cells" using a light microscope); physicochemical: immunoturbidimetric determining complement system’s components; the vaginal secretions pH studying, spectrophotometric determination and registration of ELISA results; immunological (determination of phagocytic activity of human blood neutrophils and vaginal secretions, FAL index, levels of immunoglobulins and lysozyme in blood and vaginal secretions, study of lymphocyte fractions; immunobiological (study of hormone levels in the blood, determination of the concentration of gamma-interferon, interleukins, other cytokines: TNFα, TGF-1β , in blood and vaginal secretion); molecular genetic (study of the species composition of the vaginal microbiocenosis, identification of vaginal microflora and determination of the number of genome equivalents using Real-time PCR); analytical and medical statistics. Equipment: vacuum system type "Vacutiner", centrifuge K23 (Germany), four-channel amplifier "Terсyk" (RF), fluorescent detector "Gin" RF), PCR device DTLite (RF), spectrophotometer SPECORD - 200 (Germany), tablet reader "Multiscan EX" (Finland), turbidimeter "2100 N IS" (USA), microscope «Granum» (Ukraine). Dissertation includes the theoretical generalization and experimental-clinical substantiation of the decision of actual scientific problems, namely improving the efficiency of diagnosis and accuracy of predicting the development of bacterial vaginosis by determining the impact of neurohumoral regulation, immune status, and local factors of colonization resistance to vaginal microbiocenosis. Laboratory criteria for the clinical diagnosis of vaginal dysbiosis are recommended. The extent of implementation: 7 acts of implementation, 1 patent. The field (industry) of use: healthcare (medicine: microbiology, laboratory diagnostics, and gynecology).