Sorokulova I. Theoretical basis and practice for use of the Bacillus genus bacteria for the construction of new probiotics

Aerob spore-forming bacteria of the Bacillus genus. The dissertation is devoted to elaboration of principally new probiotics on the base of the aerob spore-forming bacteria of the Bacillus genus. Methods of investigations are microbiological, biochemical, genetics, immunological. New approaches to the creation of biopreparations with unique properties were theoretically based and practically carried out. The possibility to construct the recombinant probiotics with planned properties by the methods of genetical engineering has been proved. Preclinical safety evaluation of new probiotics has been realized. The high efficacy of probiotics has been shown in experi-mental bacterial and viral infections and during their use in medicine and veterinary. New aspects of probiotic action have been discovered. The tech-nology of production and the methods of the biopreparations control were elaborated. All necessary documentation for the production of probiotics - biosporin and subalin - was maintained.