Nosenko N. Mechanisms of hormone-mediator imprinting of neuroendocrine regulation of reproduction and stress-reactivity stress-reactivity.

Object: the brain and endocrine glands of male and female rats of different age and disturbances of brain sexual differentiation. The work is aimed on studying of the imprinting mechanisms of neuroendocrine regulation of reproduction and stress-reactivity in normal subjects and its modifications by stress and hormonal influences in fetuses and newborn pups. Methods and apparatus: physiological, biochemical, morphological methods, enzyme immunoassay; spectrofluorometer, liquid chromatograph, immuno-enzymatic reader, b-spectrometer, microscope. Theoretical and practical results: the role of steroid hormones and brain biogenic monoamines in formation of disorders of reproductive functions and stress-reactivity as a result of hormonal and stress effects on the developing brain in perinatal ontogenesis has been established. The results represent a theoretical background for prevention of neuroendocrine disturbances. The new: a new neurochemical concept of hormone-mediator imprinting of neuroendocrine syste m is proposed. Disturbances of imprinting mechanisms determine long-term changes in neuroendocrine regulation of reproduction and stress-reactivity. The level of approach: recommendations for the antenatal protection of the offspring reproductive health. Field of application: endocrinology, gynecology, geronto-logy.