Didenko N. Molecular taxonomy of Centanrea L. rare species from Phalolepis (Cass.) Dobrocz. and Pseudophalolepis Klok. sections of flora of Ukraine.

Українська версія

Thesis for the degree of Doctor of Philosophy (PhD)

State registration number

0821U100221

Applicant for

Specialization

  • 091 - Біологія. Біологія

03-02-2021

Specialized Academic Board

ДФ 26.001.080

Taras Shevchenko National University of Kyiv

Essay

taxonomic status species of Centaurea L. genus from Phalolepis (Cass.) Dobrocz. and Pseudophalolepis Klok. sections of Ukraine flora based on an integrated approach with priority use of molecular phylogenetic analysis methods. The work is based on analysis of 96 ITS1-5,8S-ITS2 rDNA sequences (hereinafter - ITSs) (71 sequences were obtained by the author) and 47 sequences chloroplast, rpl32-tmL intergenic spacer (hereinafter - tmL) DNA regions (35 were obtained by the author) 27 species of knapweeds Phalolepis section (C. iljiniana Шаг., C. longiaristata Шаг., C. nikitensis Шаг., C. sarandinakiae Шаг., C. semijusta Juz., C. stankovii Шаг., C. sterilis Stev., C. vankovii Klok.), Pseudophalolepis section (C. appendicata Klok., C. breviceps Iljin., C. donetzica Klok., C. hypanica Pacz., C. konkae Klok., C. margarita-alba Klok., C. margaritacea Ten., C. paczoskii Kotov ex Klok., C. protogerberi Klok., C. protomargaritacea Klok., C. pseudoleucolepis Kleop.) and Acrolophus (Cass.) Dobrocz. subgenus (C. borysthenica Gruner, C. caprina ’ Stev., C. diffusa Lam., C. odessana Prodan, C. steveniana Klok., C. stoebe L.) and Lopholoma (Cass.) Dobrocz. subgenus (C. salonitana Vis., C. adpressa Ledeb.). 99 species and intraspecific taxa (hereinafter - operational taxonomic units, or OTU) were analyzed that fully represent the study group within Ukraine. The molecular genetic data of ITSs and trnL sequences of 8 taxa determined for the first time.

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