The dissertation work is devoted to the experimental substantiation of research of influence of drugs on structure of reproductive organs of experimental animals (white laboratory rats), namely: angioarchitectonics, micro- and ultrastructure of the uterus, to develop methods for the prevention of pathology of the female reproductive system caused by the use of narcotic drugs.
The regularities of morphological changes of uterine walls under conditions of long — term exposure to nalbuphine have been established.
Further study for 6 weeks showed a decrease in Arteriolar-to-Venular Ratio to 0. 95±0. 07 due to the expansion of the venular component of the hemomicrocirculatory tract, the density of the network of exchange vessels decreased to 14.0±2.2, and the index of trophic activity of the tissue increased significantly and amounted to (29. 03±8. 18) μm, which confirmed the destruction of capillaries.
After 6 weeks at the microstructural level in the glands of the uterine horns, alternative changes in the epithelium were more pronounced, cells with vacuolated cytoplasm, necrotized epithelial cells occured more common. The number of fibroblasts and collagen fibers increased around the terminal glands and excretory ducts. The stroma of the lamina propria of the mucosal membrane of the uterine horns was infiltrated with neutrophils, lymphocytes and macrophages. Necrotic changes in smooth myocytes also developed. Macrophages containing a golden-brown pigment – hemosiderin – were located around the hyperemic vessels.
During 6 weeks of nalbuphine administration profound changes occur in the ultrastructural organization of all membranes of uterine wall of white laboratory rats. After 6 weeks of experimental exposure to nalbuphine, the lumen of the microvessels is full of blood elements, sometimes showing adhesion of erythrocytes to the endothelium of the arterioles, lumens of the capillaries had a star-shaped, there were, characteristically, deep protrusions of the cytoplasm of the endothelium, exfoliation of the endothelial cell or its fragment in the lumen of the microvessels. Endometrial epitheliocytes were in a state of vacuolar dystrophy or with signs of necrosis. The cytoplasm was enlightened, the organelles were destructed, especially the destroyed mitochondrial apparatus, mitochondria lost their ridges, turned into microcysts. Electron microscopic examination of the lamina propria of the mucous membrane of the uterus of a white rat revealed its loosening, loss of clear contours, and sometimes discontinuity. Developed necrotic changes of smooth myocytes. More often neutrophils and macrophages accumulated near necrotized smooth myocytes. Loosening, thickening, edema of connective tissue layers between bundles of smooth myocytes of the myometrium, thickening of collagen fibers, increase in the number of fibroblasts have been established, which indicates the development of fibrosis.
The changes described above under the influence of nalbuphine indicate the development of changes in the uterine wall of degenerative-destructive nature, which may leads to reproductive health problems. The identified disorders are important for the development of methods for the prevention of pathology of the female reproductive system caused by the use of narcotic drugs.
