Fokina A. Biotechnology of microclonal propagation of Origanum vulgare L. та Paulownia elongata S.Y.Hu ⤬ P.fortunei (Seem.) Hemsl

In the dissertation research the results of the development of biotechnologies of microclonal reproduction in in vitro culture of two plants – oregano (Origanum vulgare L.) and interspecific hybrid of Paulownia (Paulownia elongata S.Y.Hu ⤬ P.fortunei (Seem.) Hemsl.) are presented. The optimization allowed to increase the reproduction rate at the stage of introduction in in vitro culture for oregano from 5.4 ± 0.02 cuttings/explant to 7.9 ± 0.04 cuttings/explant, and in paulownia from 2.2 ± 0, 2 cuttings/explant to 3.7 ± 0.4 cuttings/explant. In O.vulgare in the first cycle of sterile cutting the reproduction rate increased to 11.5 ± 0.9 cuttings/explant, in the third cycle – to 12.9 ± 0.6 cuttings/explant, and in the seventh cycle remained at 12.7 ± 0.5 cuttings/explant. In P. elongata ⤬ P.fortunei in the second cycle of sterile cutting the reproduction coefficient increased to 6.9 ± 0.4 cuttings/explant and by the VI cycle remained at the level of 6.8 ± 0.2 cuttings/explant. The maximum values achieved by optimization of oregano are for the frequency of rooting at the stage of rhizogenesis in vitro – 100%, survival after the transfer from in vitro to ex vitro conditions in the soil of the tablets – 66.1 ± 1.3%, from tablets to pots – 95%, from pots into the field to a resident place of cultivation – 90%. The maximum values achieved by optimization of paulownia at the stage of rhizogenesis in vitro for the frequency of rooting are – 100%; survival after the transfer from in vitro to ex vitro conditions in the soil of the tablets is 92.9 ± 4.2%, from tablets to pots – 94.3 ± 5.6%, after overwintering in pots – 100%, after the transfer from pots in the field to a resident place of cultivation – 94%.