Hopta O. Microbiological substantiation of the feasibility of polymeric products for medical application impregnation by nanoparticles of silver and copper

The dissertation is devoted to the study of antimicrobial action of medical products from polymers impregnated with Ag and Cu nanoparticles, on planktonic and biofilm forms of microorganisms, causative agents of purulent inflammatory diseases and mycoses existence. In order to simplify the technological process of the production of polyethylene with antimicrobial properties and the provision of the distribution of particles of silver nanoparticles in the entire volume of the material, a study was conducted to produce polyethylene with antimicrobial properties which includes mixing of polyethylene with silver nanoparticles with granulation of extrusion mixture at a temperature of 160-1900C, thereby silver nanoparticles are evenly distributed in the melt throughout the polyethylene volume, which provides stable antimicrobial effect. For the first time, it was found that for the actions of Ag and Cu nanoparticles impregnated with polyethylene products, the ability to form biofilms is reduced in all experimental strains. For the first time a violation of the integrity of the daily formed biofilm monolayer with a decrease in the density for the action of Ag and Cu nanoparticles impregnated with the products for medical use was found: a high pressure polyethylene catheter and a non-woven material on the basis of wool and synthetic polyethylene terephthalate polyester fiber. On the basis of in-depth study, the feasibility and efficacy of Ag and Cu nanoparticles impregnation of the products of high pressure polyethylene and non-woven polymeric materials for medical use together with the use of LED radiation of red and violet spectra with the possibility of prophylaxis of purulent-inflammatory processes and mycoses was proved. Based on the determination of the ability to form biofilms of purulent-inflammatory diseases causative agents in children and mycotic complications in adults with skin diseases, the microbiological justification of new approaches to complex therapy and improvement of prophylaxis methods of microbial complications are possible