The dissertation work provides a theoretical generalization and solution of a scientific task, which consists in the action of DS as an active chemical substance (CS) on the mechanisms of differentiation of stem and cells of the red sprout of the bone marrow under experimental conditions against the background of an inflammatory process (chemical dermatitis) for 96 hours.
We used 44 heads of white laboratory mice, males, weighing 18-27 g. The animals were divided into three experimental groups, acting CS - DS, was injected intramuscularly at a dose of 1.5 mg/kg (the first group consisted of 16 individuals) and 3 mg/kg (the second group of 16 individuals), the control group of animals (the third group, 12 animals), received a physiological solution of sodium chloride, the calculation of the introduction of the amount of CS DS was carried out according to the Rybolovlev formula. The study was carried out against the background of the development of experimental dermatitis caused by the method of Yamomoto.
The following methods were used in the work: experimental (modeling the effect of DS on the body against the background of the development of an inflammatory process in the form of chemical dermatitis, the main task of the study was its effect on the bone marrow), histological (preparation of bone marrow smears and their staining), morphological and physiological ( counting the number of cells of the bone marrow and their characteristics), enzyme-linked immunosorbent assay (determination of the concentration of Antigen-1 (LFA-1) in tissue homogenates, spectrophotometric determination of the concentration of lipopolysaccharides in tissue homogenates), immunophenotypic with monoclonal antibodies (immunomagnetic separation of bone marrow cells into individual cellular groups) and mathematical and statistical research methods.
It was found that the use of DN at a dose of 1.5 mg/kg in mice of the first group was not accompanied by significant changes in the total number of red bone marrow cells. Against this background, there was an increase in the number of undifferentiated blasts, which can be regarded as a compensatory response of the bone marrow to the action of an exogenous factor or by blocking factors that contribute to the transformation of cells into more mature forms.
After the enzyme-linked immunosorbent assay study, an increase in the concentration of lipopolysaccharides in the homogenates of the studied tissues was established. Lipopolysaccharides are a major membrane component of gram-negative bacteria and are responsible for triggering pro-inflammatory cytokines.
This happens when due to the strengthening of the immune system and the anti-inflammatory effect of DS on the body. By blocking the colony-stimulating factor and interleukin-3, the process of differentiation of young forms into more mature ones was inhibited. A decrease in the number of mitoses of myeloid cells, which are the direct precursors of neutrophils and monocytes, has been established. The cause of this condition is blocking of interleukins and colony-stimulating factors specific for each germ. As a result, there was a decrease in myeloocyte count and neutrophil maturation index, which is the ratio of mature to young forms of cells.
In the second group, after carrying out immunomagnetic separation and counting the number of cells in the Goryaev camera, it was found that the use of active CS DS caused the inhibition of all groups of the studied cells. There was a blockage of factors that are responsible for the formation of mature cell forms and granulocyte-macrophage colony-stimulating factor - with the direct participation of which the formation of myeloblast occurs from the cell of its myelomonoblast precursor. These tendencies testify in favor of the intoxication of the body with an increase in the amount of the active chemical substance CS introduced.
After carrying out immunomagnetic separation and counting the number of cells in the Goryaev camera, it was found that under the influence of CS DS there was an increase in the number of nuclear forms of erythropoiesis cells in both groups. In mice of the first and second groups, an increase in the number of erythroblasts was observed, the number of reticular cells decreased, the index of maturation of erythrokaryocytes, which is the ratio of mature forms of erythropoiesis cells to young, leuko-erythrokaryoid ratio (the ratio of leukopoiesis elements to the number of erythropoiesis elements), primarily for by reducing the mature forms of erythroid cells and leukocytes. There is an accumulation of nuclear forms of erythrocytes due to a decrease in the concentration of erythropoietin, prostaglandins, interleukin-3 under the influence of CS DS. The formation of mature forms of erythrocytes also occurs due to substances formed as the end product of the breakdown of erythrocytes in the spleen.
