Boichuk M. Clinical and diagnostic features of multiple sclerosis course taking into account the glycosylation of blood immunoglobulin molecules

The objects – multiple sclerosis depending on the features of glycosylation of blood immunoglobulin molecules; the purpose – Improving the diagnosis and optimization of treatment of patients with multiple sclerosis on the basis of a comprehensive clinical, neurological and immunobiological study of glycosyl determinants of serum IgG as diagnostic markers in multiple sclerosis in different conditions of the disease and treatment strategies; methods – anamnestic, clinical-neurological, immunobiological (determination of total content of IgG immunoglobulins and associated immune complexes in blood serum samples of patients with MS, determination of antibodies to basic myelin protein (anti-OBM), determination of antibodies for secondary necrotic cells, lectin-enzyme-linked immunosorbent assay for glycosylation, immunoglobulin glycosylation by capillary electrophoresis - laser-induced fluorescence, determination of neutrophil elastase in serum), statistical processing of results; results – In the dissertation work on the basis of complex clinical-neurological and immunobiological researches the new approach on improvement of MS diagnostics and definition of new prognostic biomarkers of this disease for the purpose of optimization of medical process in the conditions of neurological departments is presented. The cause of changes in glycosyl determinants in MS has been clarified - they have been shown to be associated with the appearance of circulating neutrophilic extracellular traps in the bloodstream. The use of detection of exposure to glycosyl determinants on IgG molecules as a biomarker of MS is proposed. This opens up opportunities for a better understanding of the pathogenesis in the disease, an objective assessment of the features of its clinical course, the correct prediction and selection of a correct and effective treatment strategy.