Yavorska N. Biologically active substances of highbush blueberry (Vaccinium corymbosum L.) shoots and their effect on microbiota and immune system

The dissertation is devoted to the research of peculiarities of accumulation of biologically active substances in shoots of Vaccinium corymbosum L. of varieties Bluejay, Bluecrop, Elliott, which differ in terms of phenological phases and their influence on opportunistic strains of bacteria and Candida and immune system. Studies of flavonoids, proanthocyanidins, tannins, ascorbic, organic and hydroxycinnamic acids, chlorophyll, carotenoids, elements, arbutin contents and studied the peculiarities of their accumulation at the stages of flowering, fruiting, post fruiting and in preparation for winter. The antibacterial activity of shoot extracts against Escherichia coli, Pseudomonas fluorescens, Proteus vulgaris, Bacillus subtilis, Micrococcus luteus, Staphylococcus albus was studied. Extracts made from 60-80% aqueous ethanol had high antibacterial activity against all studied strains. The anticandidal activity of extracts of V. corymbosum shoots against Candida pseudotropicalis, Candida curvata, Candida kefir, Candida parapsilosis, Candida tenuis was studied. Aqueous-ethanol extracts of Elliott shoots had high activity against Candida spp. and higher compared to extracts of Bluecrop and Bluejay. There was a shift in the expression of immunological homeostasis of the Guinea pigs under the action of water-ethanol and aqueous extracts of Elliott shoots, significant changes in B-cell immunity and humoral immunity, indicating the development of type 1 hypersensitivity reactions.