Konechnyi Y. Species spectrum and biological properties of bacterial and fungal causative agents of health-care-associated infections and study of antimicrobial activity against them using 4-thiazolidinone derivatives

The objects – healthcare associated infections (HAIs), the effect of 4-thiazolidinone derivatives on the HAIs pathogens; the purpose – optimization of methods of microbiological diagnosis of HAIs, by applying clinical-microbiological and epidemiological criteria, and a comprehensive approach in establishing the etiological role of the pathogen, as well as microbiological justification for the development of a new "drug-like molecule" with effective antimicrobial activity; methods – microbiological: cultural - to isolate pure culture of HAIs pathogens; biochemical - for species identification of HAIs pathogens; clinical - (together with medical hospitals collection of anamnesis, clinical course, conducting an objective examination of patients with ICU); questionnaire - to assess the risk factors of HAIs; molecular genetic (random enhancement of polymorphic DNA (RAPD), 16sRNA-ID, PCR) - for species identification of HAIs pathogens, detection of AMP genes and determination of affinity of strains of microorganisms; mass spectrophotometric (MALDI-TOFF, principal components method (PCA)) - species identification of HAIs pathogens and determination of strain affinity; enzyme-linked immunosorbent assays - to determine the levels of cytokines in the blood of laboratory animals; microscopic (light microscopy, dark field and fluorescent) - study of biofilms; spectrophotometric - to determine the optical density of biofilms; biological - experimental study of local irritant, allergenic, anti-inflammatory action and acute toxicity of the "leader compound" in vivo; statistical analysis - processing and analysis of the results; results – the thesis presents a theoretical generalization and solution of the current scientific and practical problem of health care, as determination of the structure and distribution of Healthcare-Associated Infections (HAIs) in Ukraine in recent years, features of the species spectrum of pathogens, and the spreading of antibiotic resistance, clinical and microbiological characteristics of HAIs, including those caused by rare pathogens of HAIs, as well as a search, synthesis, screening of new chemical compounds with antimicrobial and antifungal activity in order to introduce new prototypes of antimicrobial drugs; branch – Health care.