Litovchenko O. Peculiarities in the formation of biological effects under the influence of electromagnetic radiation in conditions of experimental cold stress

The subject of the thesis is the study of biological reactions formation in the whole organism of rats under the combined influence of low frequency electromagnetic radiation (70 kHz, 600 V / m) and low temperature (4° С) in the dynamics (5, 15 and 30 days) over 30 days, 4 hours a day by physiological, morphological changes, reactivity of the immune system, redox system balance, lipid and mineral metabolism in the laboratory experiment; with a subsequent statistical-diagnosing neuro-phase DNFS system, fuzzy clustering method to determine the dominant factor in the formation of corresponding biological effects in the body on the combined influence of factors, as well as a hybrid system for assessing the informativeness of biological indices, for processing indices when determining biological effects. The obtained results showed that, under the conditions of the combined influence of factors, the biological effects were slightly modified in comparison with the isolated influence of both electromagnetic radiation and low temperature. Peculiarities of the formation of biological effects under the combined influence of factors should be considered the predominance of inhibition processes in the CNS; the effects of summation by the following indices: decrease in catalase activity and level of SH-groups, increase of atherogenic effect by indices of decrease in HDL concentration and increase in LDL and atherogenicity index were established; suppression of phagocytosis at different stages of the experiment, violation in the balance of activation of the complement system in the classical way (decrease in component C4), increase in the intensity of IgM synthesis with a decrease in IgG concentration. The used method of cluster analysis (Fuzzy-c-means) based on artificial intelligence allowed to determine the share of contribution of each of the studied factors. The low temperature was the leading factor in the combined effect, which added 60% to the overall biological effect throughout the study, against the effect of EMR, for which the contribution rate was only 40%. It was determined that an increase in the concentration of DC with a simultaneous decrease in the concentration of SH groups, an increase in VLDL and inhibition of oxygen-dependent neutrophil metabolism by the NBT-test were the leading indices in the formation of biological effects.