Panteleichuk A. Biopolymer (biodegradable) materials application in experimental traumatic brain injury

In the dissertation research a complex study of the influence of biopolymer biodegradable film materials on the regeneration of dura mater (DM) in the model of penetrating traumatic brain injury (TBI) with decompressive craniectomy was performed. The following methods were used in the study: experimental (reproduction in the experiment of penetrating trauma with decompressive craniectomy, transplantation of various types of artificial membranes), pathomorphological macroscopic (assessment of the degree of tissue adhesion and the degree of damage to the brain surface, assessment of the presence or absence of cerebrospinal fluid, rough scars or necrotic changes in tissues, assessment of signs of inflammation-local edema, tissue redness), histological method, including confocal microscopy (assessment of resorption of matrix material, local tissue reaction, vascularization, rate of regeneration, assessment of the number of newly formed collagen fibers), morphometric method (measurement of the thickness of reduced DM), infrared spectroscopy (physical molecular statistical characteristics). between experimental groups). The dissertation presents a solution of the actual problem of modern neurosurgery, namely improving the treatment of severe penetrating traumatic brain injury by developing new effective biopolymer materials for DM plasty, methods of their use to fully restore the sealing of the DM and reduce the formation of unwanted adhesive tissue. The possibility of using selected biopolymer materials for DM plasty has been experimentally substantiated, their efficiency and safety in use have been confirmed. The study expands and complements scientific data on the effect of biopolymer (biodegradable) materials on the regeneration of the dura mater. Scientific novelty of the obtained results: a new experimental model was developed to assess the regeneration of DM in severe penetrating trauma; in the study for DM plasty, new (experimentally obtained) films based on chitosan and gelatin were used; a new scale of objective assessment of the intensity of DM regeneration was applied for the first time; a new scale of objective macroscopic assessment of the severity of the cicatricial-adhesive process in the area of the trepanation window was applied for the first time; the method of laser confocal fluorescence microscopy was used to objectively estimate the amount of newly formed collagen fibers in regenerated DM for the first time; new scientific data were obtained on the possibilities of IR spectroscopy to assess the condition of regenerated DM tissues. Practical meaning. The obtained results allow to recommend the selected materials for further clinical trials and implementation in clinical practice. Their use is likely to improve the outcome of treatment of patients with penetrating traumatic brain injury.