The dissertation presents a study of the influence of hydrogen sulfide levels in the blood plasma of patients with arterial hypertension (AH) of different ages on the formation and course of this pathology.
The aim of the research was to study the effect of changes in the level of endogenous hydrogen sulfide synthesis on markers of nitric oxidative stress, clinical and functional parameters, platelet ultrastructure and changes in the mitochondrial apparatus of leukocytes in patients of different ages with arterial hypertension..
The first chapter discusses the literature on: the modern understanding of the mechanisms of development of hypertension; endogenous hydrogen sulfide - properties and physiological effects; modern concepts of the role of endogenous hydrogen sulfide in the development of changes in the cardiovascular system and in the mechanisms of hypertensive states; the effects of exogenous donors on the formation of endogenous hydrogen sulfide.
The second chapter of the dissertation discusses the following issues: the research object is determined, the design of the clinical examination, the methods of general clinical examination, the methods of studying biochemical indicators, the preparation of preparations for electron microscopic study of the ultrastructure of blood cells (leukocytes and platelets), morphometric study of the ultrastructure of blood cells, statistical processing of results.
This section discusses: changes in the content of endogenous hydrogen sulfide in the plasma of patients of different ages with stage II at different durations of hydrogen sulfide donor application ; hydrogen sulfide content in the plasma of younger age group patients with hypertension after hydrogen sulfide donor application, hydrogen sulfide content in the plasma of older age group patients with hypertension after application donor hydrogen sulfide, features changes markers of oxidative-nitrosative stress in the plasma of patients of different ages after3 the use of hydrogen sulfide donor, markers in patients with hypertension of younger age group after the use of H2S donor, markers in patients with hypertension of older age group after the use of H2S donor, the effect of different duration of hydrogen sulfide donor application on the ultrastructure of blood cells in patients with stage II depending on the age of the examined, the ultrastructure of platelets in patients of different ages with stage II, changes in the ultrastructure of the mitochondrial apparatus of leukocytes in patients with stage II under the influence of hydrogen sulfide donor, changes in laboratory and functional indicators in patients with hypertension in different age groups under the influence of hydrogen sulfide donor, the effect of hydrogen sulfide donor on laboratory indicators and functional parameters of the cardiovascular system in patients of younger age group with hypertension, changes in laboratory indicators in patients with stage II, changes in functional indicators of the cardiovascular system in patients with stage II, the effect of hydrogen sulfide donor on laboratory indicators and functional parameters of the cardiovascular system in patients with stage II, changes in blood laboratory indicators, changes in functional indicators of the cardiovascular system, the effect of PLP on the hydrogen sulfide content in blood plasma and functional parameters in patients with stage II of the older age group.
We have shown that the use of a phytopreparation by patients with hypertension gradually increased the hydrogen sulfide content in the blood plasma, as a trend, on the 14th day. On the 30th day, this value was significantly higher than before treatment: the use of the phytopreparation for 1 month in young patients led to an increase in hydrogen sulfide concentration by 29.9% compared to the initial values before treatment, in older patients, the increase in hydrogen sulfide concentration was 34.7%, that was more significant. Thus, this phytopreparation changes the level of H2S in the plasma not only depending on the duration of its use, but also on the age of the patients.
The results obtained in this work are of fundamental importance, the fragments of expansion are the discovery of the involvement of H2S in the mechanisms of development and go through arterial drugs, and applied value. The data presented in the dissertation study show a significant normalization of the content of hydrogen sulfide in the blood plasma of patients with hypertension, improvement of functional and clinical laboratory parameters, reduction of markers of oxidative-nitrosative stress, improvement of platelet ultrastructure and significant normalization of mitochondrial apparatus of leukocytes under the influence of phytopreparation - hydrogen sulfide donor.
