Wang N. The effect of C60 fullerenes on the dynamics of musculus soleus contraction in rats after chronic alcoholisation

Українська версія

Thesis for the degree of Doctor of Philosophy (PhD)

State registration number

0824U002891

Thesis Registration Form

0824U002891.pdf

Applicant for

Specialization

  • 091 - Біологія

Specialized Academic Board

ДФ 6455

Lesya Ukrainka Volyn National University

Essay

In this work, the influence of water-soluble C60 fullerenes on the dynamic parameters of skeletal muscle contraction in rats after 3, 6 and 9 months of chronic alcoholisation was investigated. Changes in these parameters during the recovery process, 1 and 2 months after cessation of alcohol consumption were studied. In the analysis of the contraction mechanokinetics, the main biomechanical markers of the contractile process, were evaluated: the time of fatigue development in the studied muscles, the level of minimum and maximum contraction force, the level of muscle force impulse (as an analogue of muscle power). The optimal dose of water-soluble C60 fullerene was determined, which showed the most significant positive effects on the development of pathological changes in muscle dynamics in the development of alcoholic myopathy. An increase in muscle strength performance by 40-45% was recorded in comparison with rats of the "alcohol" group. It has been shown that in the development of chronic alcoholic myopathy, the use of water-soluble C60 fullerene at an optimal dose of 1 mg/kg improves the processes of restoring the mechanokinetics of contraction after cessation of alcohol consumption. All the described changes are confirmed by the analysis of biomechanical plasma markers responsible for the quality of the contractile process. The study revealed the optimal scheme for the use of water-soluble C60 fullerene to obtain the most significant positive results. It is proved that the use of C60 fullerene together with alcohol leads to a 12-15% reduction in the negative effects of chronic alcohol intoxication compared to the scheme of administering fullerene one hour after drinking alcohol or one hour before it is administered. It was proved that the scheme of co-administration of alcohol and C60fullerene leads to an increase in the time of onset of fatigue processes in the development of chronic alcoholic myopathy, and improves the quality of recovery of skeletal muscle contraction mechanokinetics and is effective in the case of prolonged development of alcoholic myopathy. The results obtained are the basis for further development of innovative pharmacological agents based on C60 fullerenes to improve rehabilitation procedures for myopathic disorders caused by alcohol intoxication.

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Research papers

1. Ноздренко Д. М., Ван Нань, Мотузюк О. П., Виговська О. В., Богуцька К. І., Нурищенко Н.Є., Прилуцький Ю. І. Аналіза скорочення скелетних м’язів хронічноалкоголізованих щурів за дії водорозчинного С60-фуллерену. Наносистеми, наноматеріали, нанотехнології Nanosistemi, Nanomateriali, Nanotehnologii. 2021, т. 19, № 2, сс. 475–485. doi.org/10.15407/nnn.19.02.475

2. Ноздренко Д. М., Ван Нань, Мотузюк О. П., Виговська О. В., Богуцька К. І., Дрозд П.Ю., Прилуцький Ю. І. Використання стимуляційних маркерів для аналізи терапевтичного впливу С60-фуллерену на динаміку м’язового скорочення хронічно-алкоголізованих щурів . Наносистеми, наноматеріали, нанотехнології - Nanosistemi, Nanomateriali, Nanotehnologii. 2021, 19 (3). С. 767-777. doi.org/10.15407/nnn.19.03.767

3. Wang Nan, Motuziuk O., Mishchenko I., Zhou Yingting. Levels of Relevant Inflammatory Factors and Oxidative Stress Markers in Alcoholic Myopathy：a Meta-analysis. Chinese General Practice. 2022, Vol. 25. (33). Pp. 4123-4129. DOI: 10.12114/j.issn.1007-9572.2022.

4. Nozdrenko D., Wang Nan, Motuziuk O., Prylutska S., Vygovska О., Bogutska K., Dmytrotsa O., Lynchak O., Prylutskyy Yu. The effect of C60-fullerenes on the recovery of musculus soleus contraction dynamics in rats after chronic alcoholization. • Біологічні Студії / Studia Biologica . 2024 . Т. 18 / No 1 С. 43–56 Doi: https://doi.org/10.30970/sbi.1801.755

5. Wang Nan, Motuziu O., Mischenko I. A review of several myopathy related to mitochondrial dysfunction. Нотатки сучасної біології. 2021. 2. Pp. 75-81. https://doi.org/10.29038/NCBio.21.2.45-54

6. Wang Nan, Motuziuk O. The Bibliometric Analysis of the Alcohol-induced Muscle Disease Based on CiteSpace. Науковий вісник Східноєвропейського національного університету імені Лесі Українки Серія: Біологічні науки, 2020, 2 (389), С. 83-91.

7. Ван Нань, Мотузюк O., Ноздренко O. Вплив С60 фулеренів на динаміку скорочення muscle soleus хронічно алкоголізованих щурів. Proceedings of the 9th International Scientific and Practical Conference «Global and Regional Aspects of Sustainable Development» (October 6-8, 2023). Copenhagen, Denmark. C. 137-138.

8. Wang Nan , Motuziuk O., Nozdrenko O. Exploring the potential of water-soluble fullerene derivative C60-PDA-GSH for cellular protection against oxidative stress: design, synthesis, characterization, and performance analysis. The 6th International scientific and practical conference “Modern research in science and education” (February 8-10, 2024) BoScience Publisher, Chicago, USA. 2024. P 39-40.

9. Wang Nan, Motuziuk O., Nozdrenko O. The therapeutic potential of C60 fullerene water solution under conditions of alcoholic myopathy. The 6th International scientific and practical conference “Global science: prospects and innovations” (February 1-3, 2024) Cognum Publishing House, Liverpool, United Kingdom. 2024. p. 26-30.

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