Zhao X. Advances in research on mechanism and function of antimicrobial peptides

In the dissertation work on the basis of research are substantiated the bactericidal mechanism, barrier protection, anti-inflammatory effect and the relationship between the anti-inflammatory effect and intestinal flora of antimicrobial peptide to develop and applicant drugs for prevention inflammation, maintain intestinal flora balance and improve growth performance. Though antibiotics have good antibacterial and anti-inflammatory effects, they may damage the intestinal barrier function, disrupt the intestinal microbial balance, and disrupt the body's intestinal homeostasis. The problems caused by the long-term large-scale use of antibiotics are becoming more and more serious, and the development of efficient and safe antibiotic substitutes is imminent. Antimicrobial peptides have attracted attention due to their broad-spectrum antimicrobial activity, not have drug resistance, and extensive biological functions. During our resserch we investigated mechanism of the antimicrobial peptide mastoparan X in killing Gram-negative bacteria in vitro and its anti-inflammatory and barrier repair functions in pneumonia and enteritis diseases in vivo, and to further study the relationship between anti-inflammatory and intestinal microbes of MPX. Finally, we explored the anti-inflammatory and barrier repair functions mechanism of MPX in the intestinal epithelial cells, laying a foundation for reducing the use of antibiotics in livestock and poultry breeding, it will help provide certain theoretical and practical value for future applications in livestock and poultry.