Yanan W. Development and Preliminary Application of test Strips for the Detection of Double residues of AFLATOXIN B1 and ZEARALENONE

Based on scientific demonstration and experimental research, this thesis is devoted to studying development and preliminary application of Immunochromatography Test Strips for the Detection of Double Residues of Aflatoxin B1 and Zearalenone, to the establishment of colloidal gold immunochromatographic test strip for the determination of aflatoxin B1 (AFB1) and zearalenone (ZEN) dual residue, so as to provide technical support for the rapid detection of AFB1 and ZEN dual residue in cereal food and feed and ensure the safety of cereal food and feed. Mycotoxins widely exist in the natural environment, mainly pollute all kinds of grain food and feed, seriously threaten the safety of world food and feed, and do harm to human health and animal husbandry development, which has aroused great concern all over the world. Among the more than 400 mycotoxins reported so far, AFB1 and ZEN are the two most important mycotoxins with wide pollution and great harm. Aflatoxins (AFs) are a toxic secondary metabolite produced by Aspergillus, and produced under natural conditions mainly include AFB1, AFB2, AFG1 and AFG2. Nowadays, the established immunoassay methods include gold immunochromatographic assay (GICA), enzyme-linked immunosorbent assay (ELISA), fluorescence immunoassay (FIA) and immunosensor (IS), etc. In contrast, GICA technology has the advantages of fast, simple, on-site operation, multiple detection and large amount of screening samples. It has become an important research topic of AFB1 and ZEN dual residue detection, and shows a broad development prospect, which is also the purpose of this study.