Semenchuk I. The microbial biosensors for detection of anionic surfactants

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0400U000316

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

24-01-2000

Specialized Academic Board

Д 26.240.01

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

Essay

Biosensors, surfactants (SC), bacteria-degraders. Elaboration of laboratory prototype of cell-based sensors for anionic SC determination. The used methods: electrochemical (amperometric, potentiometric), extraction-photometric. The influence of sample salt concentration, pH and cell content in biomembrane on the sensor response, as well as thermo-, working and storage stabilityty, were investigated. The sensitivity of a microbial sensor dependent upon the growth's phases of the strains has been studied. The responses of sensors for 82 organic substrates, which related to different classes of xenobiotics have been spread in water reservoirs were investigated. All strains that have been investigated had high sensitivity and selectivity and may be used in biosensors for detection of SC. The model of cells sensor for detection of anionic SC for the first time was developed. Field of application - ecological monitoring, control of technological processes, laboratory using.

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