Starodub V. Development of immune sensors on the basis of porous silicon and investigation of their functional characteristics

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0400U000991

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

17-04-2000

Specialized Academic Board

Д 26.240.01

Palladin Institute of Biochemistry of the National Academy of Sciences of Ukraine

Essay

Human myoglobin, components of the cells, producing lysine. Сreation of immune sensors on the basis of porous silicon for the registration of spesific interаction antigen-antibody and demonstration of their main working characteristics. Applied methods: method Lowry, ELISA, chemical luminescent immune assay, affinity chromatography, spectroscopy. The effect of the photoluminescence extinguishing during the formation of a specific immune complex on the porous silicon surface, was established. A hypothesis about the nature of the photoluminescence was proposed. Immune sensors for the detection of myoglobin in human serum and components of the cells, producing lysine, in the air were constructed. The main working characteristics of the sensors were studied. The obtained results serve as a basis for the creation of new instrumental dyagnostical devices. The field of application - institutions with medical-biological and environmental profile.

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