Mel'nyk E. The influence of temperature and osmotic factors of medium on anionic transport in erythrocytes.

. Investigation object: human erythrocytes. Purpose: the study of cotransport of anions and protons in erythrocytes in sugar-saline-water media. Methods: spectrofluorescence, pH-method, micro-electrophoresis, microscopy. The influence of decrease of temperature up to 3°C, osmotic properties of media, inhibitor (DIDS), hemin and lanthanum on the anionic transport has been studied. The comparison of sensitivity of ingoing and outgoing streams of anions in cell under the abovementioned actions has been made. . Investigation object: human erythrocytes. Purpose: the study of cotransport of anions and protons in erythrocytes in sugar-saline-water media. Methods: spectrofluorescence, pH-method, micro-electrophoresis, microscopy. The influence of decrease of temperature up to 3°C, osmotic properties of media, inhibitor (DIDS), hemin and lanthanum on the anionic transport has been studied. The comparison of sensitivity of ingoing and outgoing streams of anions in cell under the abovementioned actions has been m ade. . Investigation object: human erythrocytes. Purpose: the study of cotransport of anions and protons in erythrocytes in sugar-saline-water media. Methods: spectrofluorescence, pH-method, micro-electrophoresis, microscopy. The influence of decrease of temperature up to 3°C, osmotic properties of media, inhibitor (DIDS), hemin and lanthanum on the anionic transport has been studied. The comparison of sensitivity of ingoing and outgoing streams of anions in cell under the abovementioned actions has been made. Obtained that cotransport of anions and protons into a cell is more sensitive to temperature decrease, hypertonic influence and hemin modification, but not to DIDS or lanthanum. Degree of application: the creation of new method of preservation.